Plant cell and tissue culture pdf
Plant Tissue Culture Media
Abstract A synthetic, chemically defined medium was developed for the initiation and growth of cell and tissue cultures of red clover Trifolium pratense L. The medium is broadly supportive and suitable for the culture of other legume species, including alfalfa Medicago sativa L. Cultural variables for red clover were defined, and it is now possible to routinely establish callus cultures of red clover from reproductive and vegetative explant tissues of both mature and immature plants. Cell suspension cultures with rapid cell division rates have been established from calli by adapting the new medium to liquid culture. Regenerated plants have been grown to maturity and were fertile. It is now possible to investigate the use of tissue and cell culture techniques in red clover breeding programs.
Recent Advances in Plant in vitro Culture. Optimal growth and morphogenesis of tissues may vary for different plants according to their nutritional requirements. Moreover, tissues from different parts of plants may also have different requirements for satisfactory growth [ 1 ]. Tissue culture media were first developed from nutrient solutions used for culturing whole plants e. Plant tissue culture media should generally contain some or all of the following components: macronutrients, micronutrients, vitamins, amino acids or nitrogen supplements, source s of carbon, undefined organic supplements, growth regulators and solidifying agents. According to the International Association for Plant Physiology, the elements in concentrations greater than 0. It should be considered that the optimum concentration of each nutrient for achieving maximum growth rates varies among species.
Front Matter. Pages i-xii. PDF · Selection of Media for Tissue and Cell Culture Pages PDF · Measurements of Viability Suitable for Plant Tissue Cultures.
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